1AHF
ASPARTATE AMINOTRANSFERASE HEXAMUTANT
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.920, 79.390, 88.800 |
Unit cell angles | 90.00, 118.99, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.232 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.300 |
Data reduction software | XDS |
Refinement software | TNT |
Data quality characteristics
Overall | |
Number of reflections | 46105 * |
Completeness [%] | 97.3 * |
Redundancy | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | |
3 | 1 | drop | PLP | 0.01 (mM) | |
4 | 1 | drop | EDTA | 2 (mM) | |
5 | 1 | drop | DTT | 0.5 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.90-1.95 (M) | |
7 | 1 | reservoir | N-methylmorpholine | 200 (mM) | |
8 | 1 | reservoir | PEG400 | 2 (%) |