1AHC

THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	131.300, 131.300, 40.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 2.000
R-factor	0.18
Rwork	0.180
RMSD bond length	0.013
RMSD bond angle	2.570
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.066 *	0.129 *
Total number of observations	16246 *
Number of reflections	14030 *	2248 *
Completeness [%]	92.9 *	80.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.65 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (%(w/v))
2	1	drop	Tris-HCl	0.05 (M)
3	1	drop	protein	4 (%(w/v))
4	1	reservoir		23 (%(w/v))