1AHA

THE N-GLYCOSIDASE MECHANISM OF RIBOSOME-INACTIVATING PROTEINS IMPLIED BY CRYSTAL STRUCTURES OF ALPHA-MOMORCHARIN

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	132.200, 132.200, 38.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 * - 2.200
R-factor	0.183
Rwork	0.183
RMSD bond length	0.014
RMSD bond angle	2.830
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.087 *	0.151 *
Total number of observations	10629 *
Number of reflections	9071 *	1499 *
Completeness [%]	84.4 *	58.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.65 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		10 (%(w/v))
2	1	drop	Tris-HCl	0.05 (M)
3	1	drop	protein	4 (%(w/v))
4	1	reservoir		23 (%(w/v))