1AGQ
GLIAL CELL-DERIVED NEUROTROPHIC FACTOR FROM RAT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-02 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.850, 67.550, 71.400 |
| Unit cell angles | 90.00, 115.94, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23500 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | XSIGHT |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | |
| Number of reflections | 46219 | |
| <I/σ(I)> | 14.9 | 5 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 6.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 20% PEG 6000, 0.8 M LICL, PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.1 (mg/ml) | |
| 2 | 1 | drop | HEPES | 30 (mM) | |
| 3 | 1 | drop | sodium azide | 0.012 (%(w/v)) | |
| 4 | 1 | drop | benzamidine | 0.06 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 8 (%(w/v)) | |
| 6 | 1 | reservoir | 0.32 (M) |
