1AGL
STRUCTURE OF A DNA-BISDAUNOMYCIN COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 294 |
Detector technology | AREA DETECTOR |
Collection date | 1996-05-01 |
Detector | SDMS |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 28.270, 28.270, 53.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.31400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | DDF045 |
RMSD bond length | 0.014 |
RMSD bond angle | 3.100 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.070 |
Total number of observations | 11192 * |
Number of reflections | 2058 |
<I/σ(I)> | 5.5 |
Completeness [%] | 93.0 |
Redundancy | 6.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | pH 6.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | SPERMINE_HCL | ||
6 | 1 | 2 | WATER | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | d(CGATCG) | 0.9 (mM) | |
2 | 1 | drop | sodium cacodylate | 36 (mM) | |
3 | 1 | drop | 2.8 (mM) | ||
4 | 1 | drop | MPD | 5 (%) | |
5 | 1 | drop | spermine tetrahydrochloride | 5.7 (mM) | |
6 | 1 | drop | WP631 | 0.5 (mM) | |
7 | 1 | reservoir | 30 (%) |