1AGJ
EPIDERMOLYTIC TOXIN A FROM STAPHYLOCOCCUS AUREUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.090, 66.400, 81.770 |
| Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23500 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 27.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.177 |
| Total number of observations | 226508 * | |
| Number of reflections | 57263 | 9807 * |
| <I/σ(I)> | 27.5 | 6 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 4 * | PEG 4000 18%, DMSO 1%, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18 (%) | |
| 2 | 1 | reservoir | sodium phosphate | 50 (mM) | |
| 3 | 1 | drop | protein | 17.7 (mg/l) | |
| 4 | 1 | drop | DMSO | 1ml |
