1AG8
ALDEHYDE DEHYDROGENASE FROM BOVINE MITOCHONDRIA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 122.600, 198.400, 91.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.650 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.28200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.000 * |
| Data reduction software | BIOTEX |
| Data scaling software | BIOTEX |
| Phasing software | PHASES |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.730 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.077 | 0.149 |
| Total number of observations | 213121 * | |
| Number of reflections | 55485 | |
| <I/σ(I)> | 11.3 | 4.5 |
| Completeness [%] | 83.0 | 59 |
| Redundancy | 3.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 14% PEG 8000, 100 MM MES, PH 6.5, 0.2 MM MGCL2, 1MM NAD; THEN SOAKED IN SAME SOLUTION WITHOUT NAD OR MGCL2 FOR 1 WEEK. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 100 (mM) | |
| 2 | 1 | drop | NAD+ | 1 (mM) | |
| 3 | 1 | drop | 0.2 (mM) | ||
| 4 | 1 | drop | PEG8000 | 14 (%(w/v)) | |
| 5 | 1 | drop | enzyme | 8 (mg/ml) |
