1AG6
PLASTOCYANIN FROM SPINACH
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.582, 75.582, 32.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.183 * |
| Rwork | 0.191 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1plc |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.044 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 * | 0.630 |
| Number of reflections | 11977 * | |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 99.0 * | 91.3 * |
| Redundancy | 9.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.4 | pH 4.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | 35 (%) | |
| 2 | 1 | 1 | sodium acetate | 100 (mM) | |
| 3 | 1 | 1 | 0.2-0.3 (M) |
