1AFR
STEAROYL-ACYL CARRIER PROTEIN DESATURASE FROM CASTOR SEEDS
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-04 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.200, 147.800, 198.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.400 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.28500 |
Structure solution method | SIR + NCS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.075 |
Total number of observations | 403539 * |
Number of reflections | 79674 |
Completeness [%] | 87.0 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 20 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 6-9 (mg/ml) | |
10 | 1 | reservoir | magnesium acetate | 200 (mM) | |
11 | 1 | reservoir | ammonium sulfate | 75 (mM) | |
12 | 1 | reservoir | 2 (mM) | ||
13 | 1 | reservoir | 1 (mM) | ||
14 | 1 | reservoir | beta-octylglucoside | 0.2 (%) | |
15 | 1 | reservoir | PEG4000 | 12-15 (%) | |
2 | 1 | drop | cacodylate | 0.04 (M) | |
3 | 1 | drop | magnesium acetate | 100 (mM) | |
4 | 1 | drop | ammonium sulfate | 37.5 (mM) | |
5 | 1 | drop | 1 (mM) | ||
6 | 1 | drop | 0.5 (mM) | ||
7 | 1 | drop | beta-octylglucoside | 0.1 (%) | |
8 | 1 | drop | PEG4000 | 6-7.5 (%) | |
9 | 1 | reservoir | cacodylate | 0.08 (M) |