1AFB

STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-06-17
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	80.800, 84.500, 97.100
Unit cell angles	90.00, 104.30, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.217
Rwork	0.217
R-free	0.26100
RMSD bond length	0.007
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.54)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900	1.900 *
Rmerge	0.046	0.223 *
Number of reflections	47904
Completeness [%]	97.2	85 *
Redundancy	3	1.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	20 *	pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop		10 (mM)
3	1	drop		10 (mM)
4	1	drop	MPD	20 (%)
5	1	reservoir	PEG8000	12-15 (%)
6	1	reservoir	Tris-HCl	100 (mM)
7	1	reservoir		20 (mM)
8	1	reservoir		0.02 (%)