1AF7

CHER FROM SALMONELLA TYPHIMURIUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1995-01
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	55.450, 47.960, 63.060
Unit cell angles	90.00, 112.64, 90.00

Refinement procedure

Resolution	34.300 - 2.000
R-factor	0.196 *
Rwork	0.200
R-free	0.28000
Structure solution method	MIR
RMSD bond length	0.014 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	PHASES

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.300	2.250
High resolution limit [Å]	2.060	2.060
Rmerge	0.049	0.013
Number of reflections	18331
<I/σ(I)>	11	6.4
Completeness [%]	93.0	91.8
Redundancy	2.1	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6		MICROSEEDING; 1.2 M AMMONIUM SULFATE, 2% PEG 400, 25 MM SODIUM CITRATE PH 5.6. PRIOR TO DATA COLLECTION CRYSTAL WAS SOAKED IN THE SAME SOLUTION WITH PH ADJUSTED TO 7.0.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.2 (M)
2	1	reservoir	PEG400	2 (%)
3	1	reservoir	sodium citrate	25 (mM)