1AF5

GROUP I MOBILE INTRON ENDONUCLEASE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Collection date	1996-01
Spacegroup name	P 3 2 1
Unit cell lengths	78.200, 78.200, 67.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.248
Rwork	0.248
R-free	0.37300
RMSD bond length	0.013
RMSD bond angle	24.500 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.056 *
Total number of observations	25773 *
Number of reflections	4866 *
Completeness [%]	93.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2 (mg/ml)
2	1	reservoir	PEG6000	23 (%)
3	1	reservoir	citrate	0.1 (M)