1AF3
RAT BCL-XL AN APOPTOSIS INHIBITORY PROTEIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MACSCIENCE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.050, 64.050, 112.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27300 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.660 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.109 | 0.962 |
| Total number of observations | 68982 * | |
| Number of reflections | 8614 | |
| <I/σ(I)> | 6 | 2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 20 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Bcl-Xl(B) | 7 (mg/ml) | |
| 10 | 1 | reservoir | 1.4 (M) | ||
| 2 | 1 | drop | MES | 50 (mM) | |
| 3 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | ammonium sulfate | 0.7 (M) | |
| 7 | 1 | reservoir | MES | 100 (mM) | |
| 8 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 9 | 1 | reservoir | dithiothreitol | 2 (mM) |
