1AEP

MOLECULAR STRUCTURE OF AN APOLIPOPROTEIN DETERMINED AT 2.5-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	67.500, 67.500, 155.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.21
RMSD bond length	0.014
RMSD bond angle	2.130
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.042 *
Total number of observations	15665 *
Number of reflections	3927 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *		referred to J.Biol.Chem. 263.3960-3962 1988 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	ammonium sulfate	2.4 (M)
3	1	drop	phosphate	50 (mM)
4	1	reservoir	ammonium sulfate	2.8 (M)
5	1	reservoir	phosphate	50 (mM)