1AE3

MUTANT R82C OF GENE V PROTEIN (SINGLE-STRANDED DNA BINDING PROTEIN)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1996-01
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	77.140, 27.940, 42.190
Unit cell angles	90.00, 102.50, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.181
Rwork	0.181
R-free	0.28400
Structure solution method	DIFFERENCE FOURIERS
RMSD bond length	0.018
RMSD bond angle	28.600 *
Data reduction software	R-AXIS (IIC)
Data scaling software	R-AXIS (II)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.088
Number of reflections	4758
<I/σ(I)>	7.2
Completeness [%]	79.2	58.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7		R82C GVP WAS CRYSTALLIZED FROM SOLUTIONS CONTAINING 10 MG/ML PROTEIN, 35 MM TRIS (PH7.0), AND 4.7% PEG 4000 (W/V), EQUILIBRATED AGAINST 6% PEG 4000.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	35 (mM)
3	1	drop	PEG4000	4.7 (%(w/v))
4	1	reservoir	PEG4000	6 (%(w/v))