1ADQ
CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Collection date | 1994-06 |
Detector | RIGAKU RAXIS II |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.350, 81.950, 64.240 |
Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.310 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.113 | 0.265 |
Number of reflections | 14109 | |
<I/σ(I)> | 5.3 | 2.8 |
Completeness [%] | 98.3 | 98.8 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 17.5-21.5 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mg/ml) | |
2 | 1 | drop | 0.1 (%) | ||
3 | 1 | drop | Tris-HCl | 20 (mM) | |
4 | 1 | reservoir | PEG8000 | 17.5-22.5 (%(w/v)) | |
5 | 1 | reservoir | 0.1 (%) | ||
6 | 1 | reservoir | Tris-HCl | 100 (mM) |