1ADL

ADIPOCYTE LIPID BINDING PROTEIN COMPLEXED WITH ARACHIDONIC ACID: X-RAY CRYSTALLOGRAPHIC AND TITRATION CALORIMETRY STUDIES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	120.540, 37.700, 28.570
Unit cell angles	90.00, 92.34, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.188
Rwork	0.188
R-free	0.22000
RMSD bond length	0.007
RMSD bond angle	1.390
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.040 *
Total number of observations	70836 *
Number of reflections	16949 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ethanol	1 (%(v/v))
3	1	drop	arachidonic acid
4	1	reservoir	ammonium sulfate	2.1-3.0 (M)
5	1	reservoir		2.1-3.0 (M)