1ADE

STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE PH 7 AT 25 DEGREES CELSIUS

Experimental procedure

Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	73.260, 72.180, 82.900
Unit cell angles	90.00, 108.17, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.199
Rwork	0.199
RMSD bond length	0.011
RMSD bond angle	23.900 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.068
Total number of observations	174373 *
Number of reflections	54490 *
Completeness [%]	98.0	95 *
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	20-25 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	PEG3350	24 (%(w/w))
3	1	drop	imidazole	100 (mM)
5	1	reservoir	PEG3350