1AD9

IGG-FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY CTM01

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	81.390, 104.630, 119.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.233
Rwork	0.233
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ad0
RMSD bond length	0.008
RMSD bond angle	27.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.020
High resolution limit [Å]	2.800	2.800
Rmerge	0.105 *	0.377 *
Total number of observations	143861 *
Number of reflections	25776
<I/σ(I)>	17.2	5.3
Completeness [%]	99.9	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		13-18% PEG4000/200-250MM LISO4, BUFFERED AT PH7.6 WITH 100MM TRIS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	bis-Tris	20 (mM)
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG4000	13-18 (%)
4	1	reservoir		200-250 (mM)
5	1	reservoir	Tris	100 (mM)