1AD6

DOMAIN A OF HUMAN RETINOBLASTOMA TUMOR SUPPRESSOR

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	38.600, 68.040, 80.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
Rwork	0.201
R-free	0.24000
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.770
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.138
Number of reflections	9925
<I/σ(I)>	15	9
Completeness [%]	99.1	96.8
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0