1AD1

DIHYDROPTEROATE SYNTHETASE (APO FORM) FROM STAPHYLOCOCCUS AUREUS

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	120
Collection date	1996-08
Spacegroup name	P 1 21 1
Unit cell lengths	64.470, 42.190, 101.320
Unit cell angles	90.00, 106.33, 90.00

Refinement procedure

Resolution	13.500 - 2.200
R-factor	0.159
Rwork	0.159
R-free	0.21800
RMSD bond length	0.010
RMSD bond angle	1.400
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.500 *
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.070 *
Total number of observations	74289 *
Number of reflections	23614 *
Completeness [%]	88.0 *	69 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	12-16 (%(v/w))
2	1	reservoir	sodium potassium tartrate	200 (mM)
3	1	reservoir	bis-tris	100 (mM)