1AD0

FAB FRAGMENT OF ENGINEERED HUMAN MONOCLONAL ANTIBODY A5B7

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1995-11
Spacegroup name	P 1
Unit cell lengths	48.310, 66.270, 81.380
Unit cell angles	69.88, 82.48, 71.34

Refinement procedure

Resolution	15.000 - 2.500
R-factor	0.209
Rwork	0.209
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1clo
RMSD bond length	0.008
RMSD bond angle	27.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.690
High resolution limit [Å]	2.500	2.500
Rmerge	0.054 *	0.179 *
Total number of observations	49216 *
Number of reflections	29480
<I/σ(I)>	12.2	3.8
Completeness [%]	94.9	94
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		PEG6000/400 CACL2, BUFFERED AT PH6 WITH 40MM BIS-TRIS, pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	18 (%)
2	1	reservoir		400 (mM)
3	1	reservoir	Bis-Tris	40 (mM)
4	1	drop	Bis-Tris	20 (mM)
5	1	drop	protain	10 (mg/ml)