1ACB

CRYSTAL AND MOLECULAR STRUCTURE OF THE BOVINE ALPHA-CHYMOTRYPSIN-EGLIN C COMPLEX AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	55.300, 59.400, 42.500
Unit cell angles	90.00, 99.10, 90.00

Refinement procedure

Resolution	11.000 - 2.000
R-factor	0.167
RMSD bond length	0.018
RMSD bond angle	2.970
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	11.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.040 *
Total number of observations	31318 *
Number of reflections	16841 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *		referred to J.Mol.Biol. 208.511-513 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	alpha chymotrypsin	15 (mg/ml)
2	1	drop	eglin c	7 (mg/ml)
3	1	drop	phosphate	0.05 (M)
4	1	drop	PEG4000	5 (%(w/v))	can be replaced with PEG6000
5	1	reservoir	PEG4000	10 (%)	can be replaced with PEG6000