1AC1
DSBA MUTANT H32L
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 290 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.700, 65.100, 75.900 |
Unit cell angles | 90.00, 126.10, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.22000 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 1.7 A STRUCTURE OF WILDTYPE OXIDISED DSBA (PDB ENTRY 1FVK) |
RMSD bond length | 0.007 |
RMSD bond angle | 22.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.081 | 0.298 |
Total number of observations | 84044 * | |
Number of reflections | 28490 | |
<I/σ(I)> | 13.6 | 2.36 |
Completeness [%] | 91.5 | 83 |
Redundancy | 2.9 | 2.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | 21 * | Martin, J.L., (1993) J.Mol.Biol., 230, 1097. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
2 | 1 | reservoir | MPD | 1 (%) | |
3 | 1 | reservoir | cacodylate | 0.1 (M) | |
4 | 1 | drop | MPD | 1 (%) | |
5 | 1 | drop | PEG8000 | 20-25 (%(w/v)) | |
6 | 1 | drop | cacodylate | 0.1 (M) | |
7 | 1 | drop | DsbA solution | 0.002ml |