1ABB
CONTROL OF PHOSPHORYLASE B CONFORMATION BY A MODIFIED COFACTOR: CRYSTALLOGRAPHIC STUDIES ON R-STATE GLYCOGEN PHOSPHORYLASE RECONSTITUTED WITH PYRIDOXAL 5'-DIPHOSPHATE
Experimental procedure
Spacegroup name | P 1 21 1 |
Unit cell lengths | 119.000, 188.100, 88.100 |
Unit cell angles | 90.00, 109.29, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.21 |
Rwork | 0.210 |
RMSD bond length | 0.020 |
RMSD bond angle | 4.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.108 * |
Total number of observations | 107286 * |
Number of reflections | 78049 * |
Completeness [%] | 98.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7.5 * | 16 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 2 | ammonium sulfate | 1.2-1.4 (M) | |
3 | 1 | 2 | IMP | 6 (mM) | |
4 | 1 | 2 | beta-glycerophosphate | 10 (mM) | |
5 | 1 | 2 | EDTA | 0.5 (mM) | |
6 | 1 | 2 | dithiothreitol | 3 (mM) | |
7 | 1 | 2 | 0.02 (%) |