1ABA

THE STRUCTURE OF OXIDIZED BACTERIOPHAGE T4 GLUTAREDOXIN (THIOREDOXIN). REFINEMENT OF NATIVE AND MUTANT PROTEINS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	30.200, 47.800, 61.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.450
R-factor	0.175
Rwork	0.175
RMSD bond length	0.012
RMSD bond angle	2.530
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.450 *
Rmerge	0.052 *
Number of reflections	15227 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	5 (%(w/v))
2	1	drop	Mes	25 (mM)
3	1	drop	protein	15 (mg/ml)
4	1	reservoirs	PEG4000	10 (%(w/v))
5	1	reservoir	Mes	50 (mM)