1AAR

STRUCTURE OF A DIUBIQUITIN CONJUGATE AND A MODEL FOR INTERACTION WITH UBIQUITIN CONJUGATING ENZYME (E2)

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	32.100, 46.200, 25.400
Unit cell angles	105.90, 113.30, 69.70

Refinement procedure

Resolution	6.000 * - 2.300
R-factor	0.19
Rwork	0.190
RMSD bond length	0.020
RMSD bond angle	4.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.066 *
Total number of observations	17483 *
Number of reflections	5392 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *		4 *	using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	36-42 (%)
2	1	1	sodium citrate	0.05 (M)