1A9Z

UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GALACTOSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	120
Detector technology	AREA DETECTOR
Collection date	1998-03
Detector	SIEMENS HI-STAR
Spacegroup name	P 32 2 1
Unit cell lengths	83.900, 83.900, 108.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.188
Rwork	0.188
Structure solution method	FOURIER DIFFERENCE
RMSD bond length	0.007
RMSD bond angle	15.048 *
Data reduction software	SAINT
Data scaling software	XCALIBRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.228
Total number of observations	112562 *
Number of reflections	32710	3288 *
<I/σ(I)>	24.6	2.9
Completeness [%]	93.0	74
Redundancy	3.4	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9	4 *	CRYSTALLIZED FROM 18% PEG8000, 500MM NACL, 20MM UDP-GALACTOSE THEN SOAKED IN 25% PEG8000, 750MM NACL, 20MM UDP-GALACTOSE, 20% ETHYLENE GLYCOL, pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	reservoir	PEG8000	18-25 (%(w/v))
3	1	reservoir		400-800 (mM)
4	1	reservoir	CHES	50 (mM)