1A9Y

UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GLUCOSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	120
Detector technology	AREA DETECTOR
Collection date	1998-03
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	85.000, 85.000, 106.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.181
Rwork	0.181
Structure solution method	FOURIER DIFFERENCE
RMSD bond length	0.010
RMSD bond angle	14.903 *
Data reduction software	SAINT
Data scaling software	XCALIBRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.041	0.204
Total number of observations	84014 *
Number of reflections	37966	3706 *
<I/σ(I)>	15	1.9
Completeness [%]	91.0	74
Redundancy	2.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9	4 *	CRYSTALLIZED FROM 18% PEG8000, 500MM NACL, 20MM UDP-GLUCOSE THEN SOAKED IN 25% PEG8000, 750MM NACL, 20MM UDP-GLUCOSE, 20% ETHYLENE GLYCOL, pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35 (mg/ml)
2	1	reservoir	PEG8000	18-25 (%(w/v))
3	1	reservoir		400-800 (mM)
4	1	reservoir	CHES	50 (mM)