1A9U
THE COMPLEX STRUCTURE OF THE MAP KINASE P38/SB203580
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-11-06 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.480, 85.030, 123.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.182 |
Rwork | 0.182 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MAP KINASE P38 |
RMSD bond length | 0.009 |
RMSD bond angle | 25.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.300 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.042 * | 0.242 |
Total number of observations | 227945 * | |
Number of reflections | 19984 | |
<I/σ(I)> | 20 | 5.3 |
Completeness [%] | 92.4 | 65.6 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 | pH 7.4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
10 | 1 | reservoir | 0.2 (M) | ||
11 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | drop | benzamidine | 1 (mM) | |
6 | 1 | drop | pepstasin | 0.001 (mM) | |
7 | 1 | drop | leupeptin | 0.01 (mM) | |
8 | 1 | drop | HEPES | 25 (mM) | |
9 | 1 | reservoir | PEG8000 | 18 (%) |