1A9N
CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.370, 128.240, 66.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.380 |
R-factor | 0.282 * |
Rwork | 0.282 |
R-free | 0.32800 |
Structure solution method | SIR |
RMSD bond length | 0.010 |
RMSD bond angle | 0.033 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.900 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.061 | 0.236 |
Number of reflections | 32587 | |
<I/σ(I)> | 8.3 | 3 |
Completeness [%] | 94.7 | 74 * |
Redundancy | 4.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.3 | 50MM NACL, 9MM MGCL2, 0.25 MM SPERMINE, 0.25% N-OCTYL-BETA-D-GLUCOPYRANOSIDE, 50MM TRIS-CL PH 7.3, 1% PEG600 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | NACL | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | SPERMINE | ||
4 | 1 | 1 | N-OCTYL-BETA-D-GLUCOPYRANOSIDE | ||
5 | 1 | 1 | TRIS | ||
6 | 1 | 1 | PEG 600 | ||
7 | 1 | 2 | TRIS | ||
8 | 1 | 2 | PEG 600 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 50 (mM) | ||
2 | 1 | reservoir | 9 (mg/ml) | ||
3 | 1 | reservoir | spermine | 0.25 (mM) | |
4 | 1 | reservoir | n-octyl-beta-D-glucopyranoside | 0.25 (%) | |
5 | 1 | reservoir | Tris-Cl | 50 (mM) | |
6 | 1 | reservoir | PEG600 | 1 (%) |