1A9N
CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.370, 128.240, 66.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.380 |
| R-factor | 0.282 * |
| Rwork | 0.282 |
| R-free | 0.32800 |
| Structure solution method | SIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 2.510 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.061 | 0.236 |
| Number of reflections | 32587 | |
| <I/σ(I)> | 8.3 | 3 |
| Completeness [%] | 94.7 | 74 * |
| Redundancy | 4.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.3 | 50MM NACL, 9MM MGCL2, 0.25 MM SPERMINE, 0.25% N-OCTYL-BETA-D-GLUCOPYRANOSIDE, 50MM TRIS-CL PH 7.3, 1% PEG600 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NACL | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | N-OCTYL-BETA-D-GLUCOPYRANOSIDE | ||
| 5 | 1 | 1 | TRIS | ||
| 6 | 1 | 1 | PEG 600 | ||
| 7 | 1 | 2 | TRIS | ||
| 8 | 1 | 2 | PEG 600 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 50 (mM) | ||
| 2 | 1 | reservoir | 9 (mg/ml) | ||
| 3 | 1 | reservoir | spermine | 0.25 (mM) | |
| 4 | 1 | reservoir | n-octyl-beta-D-glucopyranoside | 0.25 (%) | |
| 5 | 1 | reservoir | Tris-Cl | 50 (mM) | |
| 6 | 1 | reservoir | PEG600 | 1 (%) |
