1A99
PUTRESCINE RECEPTOR (POTF) FROM E. COLI
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 269.400, 82.330, 93.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 * - 2.200 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.26000 * |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.490 * |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 * |
High resolution limit [Å] | 2.200 * |
Rmerge | 0.069 * |
Total number of observations | 273045 * |
Number of reflections | 87495 * |
Completeness [%] | 94.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | Vassylyev, D.G., (1998) Acta Crystallogr.,Sect.D, 54, 132. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 0.00125ml | ||
4 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
5 | 1 | reservoir | glycerol | 6 (%) | |
6 | 1 | reservoir | cacodylate | 200 (mM) |