1A99

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	269.400, 82.330, 93.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.200
R-factor	0.185
Rwork	0.185
R-free	0.26000 *
RMSD bond length	0.010 *
RMSD bond angle	1.490 *
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.069 *
Total number of observations	273045 *
Number of reflections	87495 *
Completeness [%]	94.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		Vassylyev, D.G., (1998) Acta Crystallogr.,Sect.D, 54, 132. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop			0.00125ml
4	1	reservoir	ammonium sulfate	2.5 (M)
5	1	reservoir	glycerol	6 (%)
6	1	reservoir	cacodylate	200 (mM)