1A92

OLIGOMERIZATION DOMAIN OF HEPATITIS DELTA ANTIGEN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	113
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1996-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	109.220, 85.300, 29.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.225 *
Rwork	0.227
R-free	0.27000 *
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	16.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.042	0.180 *
Total number of observations	221286 *
Number of reflections	28279
<I/σ(I)>	51	7
Completeness [%]	94.0	35 *
Redundancy	7.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	22 *	pH 5.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	15 (mg/ml)
2	1	drop	sodium acetate	100 (mM)
3	1	drop	sodium citrate	100 (mM)
4	1	reservoir	sodium acetate	100 (mM)
5	1	reservoir	sodium citrate	100 (mM)