1A8V
STRUCTURE OF THE RNA-BINDING DOMAIN OF THE RHO TRANSCRIPTION TERMINATOR
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.420, 36.160, 54.250 |
| Unit cell angles | 99.06, 102.64, 96.43 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.193 * |
| Rwork | 0.193 |
| R-free | 0.26100 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.169 |
| Number of reflections | 14432 | |
| <I/σ(I)> | 18.9 | 7 |
| Completeness [%] | 98.9 | 95.8 |
| Redundancy | 4.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 30 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 10-20 (%) | |
| 3 | 1 | reservoir | ammonium acetate | 50-100 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 5 | 1 | reservoir | 4 (mM) | ||
| 6 | 1 | reservoir | erbium acetate | 2 (mM) |
