1A8L
PROTEIN DISULFIDE OXIDOREDUCTASE FROM ARCHAEON PYROCOCCUS FURIOSUS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 110.291, 110.291, 68.514 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.21700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.050 |
| Number of reflections | 20703 | |
| <I/σ(I)> | 27 | 7 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 16 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 293 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | sodium azide | 0.01 (%) | |
| 4 | 1 | reservoir | mPEG550 | 25 (%) | |
| 5 | 1 | reservoir | zinc chloride | 0.2 (M) | |
| 6 | 1 | reservoir | bis-Tris | 0.1 (M) |
