1A8H

METHIONYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	288
Collection date	1995-04
Spacegroup name	P 21 21 21
Unit cell lengths	57.020, 82.470, 116.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.21 *
Rwork	0.205
R-free	0.29100 *
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.080 *	0.288 *
Total number of observations	146275 *
Number of reflections	34121 *
Completeness [%]	89.6 *	74.7 *
Redundancy	4.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	20 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	PEG6000	5 (%)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop	HEPES	100 (mM)
5	1	reservoir	PEG6000	25 (%)
6	1	reservoir	dithiothreitol	1 (mM)
7	1	reservoir	HEPES	100 (mM)