1A8F
HUMAN SERUM TRANSFERRIN, RECOMBINANT N-TERMINAL LOBE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1996-02 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.674, 72.674, 154.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.202 |
Rwork | 0.197 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HUMAN TRANSFERRIN |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.082 |
Number of reflections | 36895 |
<I/σ(I)> | 6.6 |
Completeness [%] | 94.0 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 4 * | PROTEIN WAS CRYSTALLIZED FROM 40% (V/V) ETHANOL WITH 50MM POTASSIUM CACODYLATE, PH 6.1. GROWN IN SITTING DROPS AT 277 K. RESERVOIRS CONTAINED 40% (V/V) ETHANOL, 50MM POTASSIUM CACODYLATE, PH 5.8 - 6.3. PRIOR TO CRYSTALLIZATION, THE PROTEIN WAS DIALYSED AGAINST SODIUM BICARBONATE, PH 8.1, vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium cacodylate | 50 (mM) | |
2 | 1 | drop | ethanol | 40 (%(v/v)) | |
3 | 1 | reservoir | ethanol | 40 (%(v/v)) | |
4 | 1 | reservoir | potassium cacodylate | 50 (mM) |