1A8A

RAT ANNEXIN V COMPLEXED WITH GLYCEROPHOSPHOSERINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-08
Detector	RIGAKU
Spacegroup name	H 3
Unit cell lengths	157.270, 157.270, 37.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.201
Rwork	0.201
Structure solution method	DIFFERENCE FOURIER
RMSD bond length	0.018
RMSD bond angle	20.900 *
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	44.700
High resolution limit [Å]	1.900
Rmerge	0.124
Total number of observations	62628 *
Number of reflections	20264
Completeness [%]	74.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *		PROTEIN CRYSTALLIZED FROM AMMONIUM SULFATE, 20MM CACL2, 50MM HEPES, PH 8.2; SOAKED IN 20% PEG, 20MM CACL2, 80MM GSE, PH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-30 (mg/ml)
2	1	reservoir	ammonium sulfate	40-60 (%)
3	1	reservoir		5-10 (mM)
4	1	reservoir	HEPES	50 (mM)
5	1	reservoir	dithiothreitol	1 (mM)
6	1	reservoir	sodium azide