1A7X

FKBP12-FK1012 COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	294
Detector technology	AREA DETECTOR
Collection date	1995-04-10
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	C 2 2 21
Unit cell lengths	133.590, 40.510, 93.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.173 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fkf
RMSD bond length	0.010
RMSD bond angle	2.800
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.046 *
Number of reflections	15896
<I/σ(I)>	14
Completeness [%]	91.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6		drop contains equal volume of the reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	sodium formate	5.1 (M)
4	1	reservoir	sodium acetate	0.1 (M)