1A7T

METALLO-BETA-LACTAMASE WITH MES

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-02-05
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	42.270, 66.960, 69.480
Unit cell angles	90.00, 103.77, 90.00

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.151 *
Rwork	0.151
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	0.035
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	PHASES
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.850	1.850
Rmerge	0.032	0.074
Number of reflections	29508	3950 *
<I/σ(I)>	42.8	6.7
Completeness [%]	91.3	73.9
Redundancy	2.06	1.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.3 *		PROTEIN WAS CRYSTALLIZED FROM 20% PEG 4000, 100 MM POTASSIUM ACETATE, 100 MM MES, PH 6.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	enzyme	10 (mg/ml)
2	1	1	sodium cacodylate	10 (mM)
3	1	1	dithiothreitol	2 (mM)
4	1	1	sodium azide	3 (mM)
5	1	2	MES	100 (mM)
6	1	2	potassium acetate	100 (mM)
7	1	2	PEG4000	18-21 (%)