1A7K
GLYCOSOMAL GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN A MONOCLINIC CRYSTAL FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-02 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.900, 81.000, 131.900 |
| Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TRYPANOSOMA BRUCEI GAPDH |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 * | 0.481 * |
| Total number of observations | 78266 * | |
| Number of reflections | 28710 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 81.0 | 81 |
| Redundancy | 2.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | VAPOR DIFFUSION: 100 MM SODIUM PHOSPHATE, 27.5% PEG-1000 PH 7.0 (PH ADJUSTED WITH 100 MM CITRIC ACID), vapor diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 2 | 1 | drop | triethanolamine | 100 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | sodium phosphate | 100 (mM) | |
| 7 | 1 | reservoir | PEG1000 | 27.5 (%) | |
| 8 | 1 | reservoir | citric acid | 100 (mM) |
