1A7K
GLYCOSOMAL GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN A MONOCLINIC CRYSTAL FORM
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10-02 |
Detector | FUJI |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.900, 81.000, 131.900 |
Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TRYPANOSOMA BRUCEI GAPDH |
RMSD bond length | 0.011 |
RMSD bond angle | 25.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.071 * | 0.481 * |
Total number of observations | 78266 * | |
Number of reflections | 28710 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 81.0 | 81 |
Redundancy | 2.5 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | VAPOR DIFFUSION: 100 MM SODIUM PHOSPHATE, 27.5% PEG-1000 PH 7.0 (PH ADJUSTED WITH 100 MM CITRIC ACID), vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | drop | triethanolamine | 100 (mM) | |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | EDTA | 1 (mM) | |
6 | 1 | reservoir | sodium phosphate | 100 (mM) | |
7 | 1 | reservoir | PEG1000 | 27.5 (%) | |
8 | 1 | reservoir | citric acid | 100 (mM) |