1A7J
PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | RIGAKU |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 129.830, 129.830, 129.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.28400 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 27.640 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.062 |
| Total number of observations | 42132 * |
| Number of reflections | 10956 |
| Completeness [%] | 80.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.2 * | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM PHOSPHATE, pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
