1A77
FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS JANNASCHII
Experimental procedure
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.830, 42.480, 62.560 |
Unit cell angles | 90.00, 92.24, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.27500 |
RMSD bond length | 0.008 |
RMSD bond angle | 25.400 * |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.045 * |
Total number of observations | 49148 * |
Number of reflections | 20663 * |
Completeness [%] | 97.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 10 (%) | |
2 | 1 | reservoir | 5 (mM) | ||
3 | 1 | reservoir | HEPES/Na | 100 (mM) |