1A75
WHITING PARVALBUMIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.730, 27.590, 98.510 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| R-factor | 0.2139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.017 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 12412 | |
| <I/σ(I)> | 8.6 | 5 |
| Completeness [%] | 82.2 | 73.2 |
| Redundancy | 1.54 | 1.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | SITTING DROP. RESERVOIR: 1 ML 2.1M NAH2PO4/NA2HPO4 (PH 6.0) 0.7M (NH4)2SO4, 0.02%(W/V) NAN3 DROP: 10 MICROL. PROTEIN SOLUTION (15MG/ML) +10 MICROL. RESERVOIR SOLUTION., vapor diffusion - sitting drop |
