1A74
I-PPOL HOMING ENDONUCLEASE/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.400, 113.400, 88.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.900 * |
| R-factor | 0.201 * |
| Rwork | 0.216 |
| R-free | 0.24200 * |
| Structure solution method | MIR |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 1.900 * | 2.200 |
| Rmerge | 0.033 * | 0.093 |
| Total number of observations | 179752 * | |
| Number of reflections | 50210 * | |
| <I/σ(I)> | 15 | 8 |
| Completeness [%] | 96.3 * | 75.8 |
| Redundancy | 3.58 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | DNA:protein solution(in 2:1 ratio) was mixed with an equal volume of reservoir solution * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | CITRATE | ||
| 3 | 1 | 1 | EDTA | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | NACL | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | I-PpoI | 3 (mg/ml) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | citrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 21-27 (%) |
