1A73
INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-12-15 |
| Detector | ADSC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.700, 113.700, 88.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.29700 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 | 0.120 |
| Total number of observations | 362336 * | |
| Number of reflections | 71892 * | |
| <I/σ(I)> | 19.1 | 4.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.04 | 4.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 4 * | DNA:protein solution(in 2:1 ratio) was mixed with an equal volume of reservoir solution, Flick, K.E., (1997) Protein Sci., 6, 2677. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | EDTA | ||
| 2 | 1 | 1 | SPERMINE | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | SODIUM CITRATE | ||
| 6 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | I-PpoI | 3 (mg/ml) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | EDTA | 2 (mM) | |
| 4 | 1 | reservoir | citrate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 21-27 (%) |
