1A70
SPINACH FERREDOXIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-06 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.000, 39.180, 83.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.700 |
R-factor | 0.19 |
Rwork | 0.182 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1frr |
RMSD bond length | 0.019 |
RMSD bond angle | 18.500 * |
Data reduction software | MOSFLM |
Data scaling software | Agrovata |
Phasing software | AMoRE |
Refinement software | TNT (5D) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 1.800 | |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.078 | 0.202 |
Total number of observations | 35600 * | |
Number of reflections | 11755 | |
<I/σ(I)> | 10 | 2 |
Completeness [%] | 97.6 | 68 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 277 * | PROTEIN WAS CRYSTALLIZED FROM 2.6M AMMONIUM SULPHATE IN 50MM PHOSPHATE BUFFER, PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 1.3 (M) | |
3 | 1 | drop | phosphate | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 2.6 (M) | |
5 | 1 | reservoir | phosphate | 50 (mM) |