1A70
SPINACH FERREDOXIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.000, 39.180, 83.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.182 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1frr |
| RMSD bond length | 0.019 |
| RMSD bond angle | 18.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | Agrovata |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.800 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.078 | 0.202 |
| Total number of observations | 35600 * | |
| Number of reflections | 11755 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 97.6 | 68 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 277 * | PROTEIN WAS CRYSTALLIZED FROM 2.6M AMMONIUM SULPHATE IN 50MM PHOSPHATE BUFFER, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.3 (M) | |
| 3 | 1 | drop | phosphate | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.6 (M) | |
| 5 | 1 | reservoir | phosphate | 50 (mM) |
