1A6Z
HFE (HUMAN) HEMOCHROMATOSIS PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.800, 100.100, 147.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2clr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 28.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.857) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.059 | 0.421 |
| Total number of observations | 188780 * | |
| Number of reflections | 32145 * | |
| <I/σ(I)> | 24 | 3 |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.9 * | drop contained 1:1 mixture of protein and reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 16 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium acetate | 0.4 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
