1A6W
B1-8 FV FRAGMENT COMPLEXED WITH A (4-HYDROXY-5-IODO-3-NITROPHENYL) ACETATE COMPOUND
Experimental procedure
| Temperature [K] | 269 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-08 |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.270, 83.510, 40.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| Rwork | 0.171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.041 |
| Data reduction software | MOSFLM |
| Data scaling software | Agrovata |
| Phasing software | MLPHARE |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.301 |
| Number of reflections | 65498 | |
| Completeness [%] | 95.1 | 79.2 |
| Redundancy | 2.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | dialysis | THE PURIFIED PROTEIN HAS BEEN DIALYSED INTO A 10 MM TRIS-HCL PH 7.5 BUFFER ALSO CONTAINING 20 MM NACL. PEG 1550 WAS USED AS PRECIPITANT., dialysis |
