1A6T

FAB FRAGMENT OF MAB1-IA MONOCLONAL ANTIBODY TO HUMAN RHINOVIRUS 14 NIM-IA SITE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 2
Unit cell lengths	92.170, 135.950, 81.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.700
R-factor	0.169
Rwork	0.169
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1for
RMSD bond length	0.010
RMSD bond angle	1.870
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.042	0.150
Number of reflections	29406
<I/σ(I)>	3700	581
Completeness [%]	91.7	89.2
Redundancy	2.4	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		SITTING DROP METHOD USING 18-22% PEG8000, 0.1 M SODIUM PHOSPHATE BUFFER (PH 6.0), AND 1% 2-METHYL-2,4-PENTANEDIOL WITH AN FAB1 CONCENTRATION OF 18 MG/ML., vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab1	18 (mg/ml)
2	1	reservoir	PEG8000	18-22 (%)
3	1	reservoir	sodium phosphate	0.1 (M)
4	1	reservoir	MPD	1 (%)